Hepatic sinusoidal endothelial cells (HSECs) are an unique sub-population of liver that lines the hepatic sinusoids. Their role involves in clearing waste products from the blood, regulating pericyte contractility and innate immune functions. The immortalized Mouse Hepatic Sinusoidal Endothelial Cells- SV40 is derived from C57BL/6 mouse HSECs that have maintained endothelial characteristics and HSEC-specific features such as vWF and caveolin-1. These cells also migrate in response to angiogenici growth factors, form vascular tube-like structures, endycytose acetylated low-density lipoprotein (AcLDL) and secrete proteins involved in matrix remodelling. In addition, these cells are easily transduced by retrovirus. The Immortalized Mouse Hepatic Sinusoidal Endothelial Cells- SV40 is therefore suitable for studying liver endothelial cell biology, including motility and angiogenesis. Immortalization Method: Serial passaging and transduction with pantropic recombinant retroviruses carrying SV40 Large T antigenBioSafety Level: IISpecies: MouseSource Organ: LiverGrowth Properties: AdherentMorphology: Cobble-stonePopulation Doubling: 22-32 hoursSeeding Density: 5,000-7,000 cells/cm²; Recommended split ratio is 1:2 to 1:3Markers: vWF and caveolin-1Donor Age: N/ADonor Gender: N/ADonor Ethnicity: N/APropagation: The base medium for this cell line is Prigrow I medium available at abm (TM001). To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 5%, Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: 1. Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.
2. Western blot was used to confirm the presence of endothelial markers such as vWF and caveolin-1.Shipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C