Immortalized Mouse Embryonic Hepatic Progenitor Cells (iHP)

The Immortalized Mouse Embryonic Hepatic Progenitor Cells are non-tumorigenic, hygromycin-resistant, reversibly immortalized cells that expresses endothelial (CD34), progenitor (Pou5f1/Oct4), and hepatic (AFP, Dlk, Alb) cell markers. The cells are programmed for liver-specific gene expression and differentiation, as indicated by the presence of hepatic/pancreatic transcriptions factors (i.e. HNF3/Foxa1, HNF3β/Foxa2, and HNF4α/MODY1) that are involved in mouse liver development. Indocyanine green (ICG) uptake and glycogen storage functions has also been observed in these cells. The cells are unique as the immortalization process may be reversed by removing SV40 large T antigen expression via transduction with recombinant adenovirus expressing Cre recombinase (abm Cat. No. 000018A), resulting in primary hepatic progenitor cells. Both immortalized and primary forms of the hepatic progenitor may undergo differentiation in vitro when cultured with dexamethasone, developing mature hepatocytes which express hepatic cell markers (i.e. CK18, TAT, and ApoB). It is recommended to use these cells for studies in liver stem cell, liver organogenesis, and hepatocytes transplant therapy.

Immortalization Method: Serial passaging and transduction with retrovirus SSR#69 expressing SV40 large T antigen flanked with Cre/loxP sites

BioSafety Level: II

Species: Mouse

Source Organ: Liver

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: 30 - 40 hours

Seeding Density: 10,000 - 30,000 cells/cm²

Markers: CD34, Pou4f1/Oct4, AFP, Dlk, Alb

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available at abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.
To differentiate cells: Add dexamethasone to a final concentration of 1 µM to the complete growth medium.
* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1) qPCR to examine expression of cell markers; 2) Western blot analysis for SV40 large T antigen; 3) Indocyanine green (ICG) uptake and release assay and periodic acid-schiff (PAS) staining were performed to determine cell functions.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C