Immortalized Mouse Cranial Suture Cells (iMSu) are immortalized through infection with non-replicating retrovirus carrying the loxP-flanked SV40 large T antigen. In vitro, the cells can be genetically manipulated for further research. Thus it is a reliable tool for research in mouse cranial suture biology and molecular signalling mechanism implicated in cranial sutures pathophysiology such as craniosynostosis.
Immortalization Method: Serial passaging and infection with retrovirus carrying loxP-flanked SV40 large T antigen
BioSafety Level: II
Species: Mouse
Source Organ: Cranium
Growth Properties: Adherent
Morphology: Polygonal
Population Doubling: 21 hours
Seeding Density: 20,000 cells/cm²
Markers: N/A
Donor Age: N/A
Donor Gender: N/A
Donor Ethnicity: N/A
Propagation: The base medium for this cell line is Prigrow III medium available at abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control: N/A
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C