In aldosterone-sensitive distal nephron (ASDN), mineralocorticoid receptor (MR) and glucocorticoid receptor (GR) activation regulate ionic and water transports through the epithelial sodium channel (ENaC) and the Na+/ K+ pump. The Immortalized Mouse Cortical Collecting Duct Cell Line is a spontaneously transformed Liddle mouse cell line that expresses significant level of functionally active MR and GR, as well as active 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2) enzyme. In addition, this cell line responses to physiological concentrations (K(1/2)= 0.3 nM) of aldosterone, making it useful for binding equilibrium assays and studies on sodium transport response to various mineralocorticoid and glucocorticoid agonists. Immortalization Method: Spontaneously immortalizationBioSafety Level: IISpecies: MouseSource Organ: KidneyGrowth Properties: AdherentMorphology: OtherPopulation Doubling: N/ASeeding Density: In early passages, the cells do not form domes and grow more slowly. Split low passage cells at 1:6 ratio. At higher passages (above p15), the cells grow more rapidly and are recommended to be split at 1:10 ratio. Markers: N/ADonor Age: N/ADonor Gender: N/ADonor Ethnicity: N/APropagation: The base medium for this cell line is Prigrow IV medium available at abm (TM004). To make the complete growth medium, add the following components to the base medium: 2% fetal calf serum, 0.87 µM insulin, 5 µg/ml human apotransferrin, 10 ng/ml EGF, 1 nM T3, 30 nM dexamethasone and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C. Quality Control: 1) Immunofluorescence and RT-PCR were used to confirm the expression of epithelial sodium channel (ENaC) and Na-K-ATPase; 2) RT-PCR was used to detect expression of MR, GR, and 11β-HSD2 ; 3) sodium transport response assayed through short-circuit current (electrophysiologic studies) and binding assays.Shipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C