Description: Immortalized Mouse Achilles Tendon Cells (iMAT) are derived from the Achilles tendon of 4-week old CD1 mice and are immortalized using the piggyBac transposon system. The cells are hygromycin resistant. Tendon cells or tenocytes are differentiated cells that form the mature tendon as it is involved in synthesizing the extracellular matrix. iMAT is a valuable tool in studies relating to the native repair mechanisms of tendons.
Immortalization Method: Serial passaging and co-transfection with piggyBac vector MPH86 expressing SV40 T Ag flanked with FLP and piggyBac transposase expression vector
BioSafety Level: II
Species: Mouse
Source Organ: Achilles tendon
Growth Properties: Adherent
Morphology: Polygonal
Population Doubling: N/A
Seeding Density: 10,000 - 30,000 cells / cm10²
Markers: N/A
Donor Age: N/A
Donor Gender: N/A
Donor Ethnicity: N/A
Propagation: The base medium for this cell line is Prigrow III medium available at
abm (
TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (
TM999)* to final concentration of 10% and Penicillin/Streptomycin Solution (
G255) to a final concentration of 1%. Carbon dioxide (CO
2): 5%, Temperature: +37.0°C.
* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: N/A
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C