Immortalized Mouse Achilles Tendon Cells (iMAT)

Immortalized Mouse Achilles Tendon Cells (iMAT) are derived from the Achilles tendon of 4-week old CD1 mice and are immortalized using the piggyBac transposon system. The cells are hygromycin resistant. Tendon cells or tenocytes are differentiated cells that form the mature tendon as it is involved in synthesizing the extracellular matrix. iMAT is a valuable tool in studies relating to the native repair mechanisms of tendons.

Immortalization Method: Serial passaging and co-transfection with piggyBac vector MPH86 expressing SV40 T Ag flanked with FLP and piggyBac transposase expression vector

BioSafety Level: II

Species: Mouse

Source Organ: Achilles tendon

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: N/A

Seeding Density: 10,000 - 30,000 cells / cm²

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available at abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: N/A

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C