Immortalized marmoset hepatic progenitor cells (MHPCs) are generated by lentivirus-mediated transfer of the simian virus 40 large T antigen gene in fetal liver polygonal cells. These cells possess hepatic progenitor cell-specific gene expression profiles with potential to differentiate into both hepatocytic and cholangiocytic lineages
in vitro and
in vivo and also can be genetically modified. Importantly, injected MHPCs repopulated the injured liver of fumarylacetoacetate hydrolase-deficient mice with hepatocyte-like cells. MHPCs also engraft as cholangiocytes into bile ducts of 3,5-diethoxycarbonyl-1,4-dihydrocollidine-induced bile ductular injured mice. MHPCs provide a tool to enable efficient derivation and genetic modification of both hepatocytes and cholangiocytes for use in disease modeling, tissue engineering, and drug screening.
Immortalization Method: Immortalized with lentivirus containing the SV40 T antigen
BioSafety Level: II
Organism: Marmoset
Species: Marmoset
Source Organs: Liver
Organ Type: Liver
Growth Properties: Adherent
Morphology: Elongated spindle
Population Doubling: 55 hours
Markers: Hepatic progenitor markers (CD44, Sox9, Sox17 and Ncam1)
Propagation: The base culture medium for this cell line is Prigrow IV medium available at
abm, Cat. No.
TM004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, Penicillin/Streptomycin Solution (G255) to a final concentration of 1%, 2-mercaptoethanol to a final concentration of 0.1 mM, 10 ng/ml hepatocyte growth factor (HGF) to a final concentration of 10 ng/ml, Recombinant Human EGF (Z100139) to a final concentration of 10 ng/ml, insulin-transferrin-selenium (Invitrogen) to a final concentration of 1X, dexamethasone (Sigma) to a final concentration of 10
-7 M, and nicotinamide (Sigma) to a final concentration of 10 ng/ml. Change media every 2-3 days.Carbon dioxide (CO
2): 5%, Temperature: +37.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control: 1) Karyotype and telomere analysis; 2) RNA sequencing; 3) Reverse transcription-PCR; 4) Immunocytochemistry and immunohistochemistry; 5) Histology; 6) PAS, ICG, and CDFDA uptake assays and Oil Red O staining; 7)
in vitro differentiation; 8) CYP450 induction
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Guo, Z., Jing, R., Rao, Q., Zhang, L., Gao, Y., Liu, F., … Yin, H. (2018). Immortalized common marmoset (Callithrix jacchus) hepatic progenitor cells possess bipotentiality
in vitro and
in vivo. Cell Discovery, 4(1). https://doi.org/10.1038/s41421-018-0020-7