Human Umbilical Vein Endothelial Cells (HUVEC) are derived from the endothelium of veins from the umbilical cord. They are used as a classic model system to study the physiological function as well as pathology of endothelial cells in many diseases such as atherosclerosis and coronary artery disease.
Immortalization Method: Spontaneous
BioSafety Level: II
Organism: Human (H. sapiens)
Species: Human
Source Organs: Reproductive
Organ Type: Reproductive
Growth Properties: Adherent
Morphology: Round
Markers: miR-216a expression
Donor Age: Not disclosed
Propagation:Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The base medium for this cell line is Endothelial Cell Medium (ScienCell, San Diego, CA, USA). To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 5% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Change media every 2-3 days.Carbon dioxide (CO2): 5%, Temperature: +37.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control: 1) Assays for proliferation, migration, angiogenic and cell adhesion activity; 2) mRNA expression analysis; 3) Telomerase enzyme activity and telomere length measurement; 4) Western blot
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Yang, S., Mi, X., Chen, Y., Feng, C., Hou, Z., Hui, R., & Zhang, W. (2018). MicroRNA-216a induces endothelial senescence and inflammation via Smad3/IκBα pathway. Journal of cellular and molecular medicine, 22(5), 2739–2749. https://doi.org/10.1111/jcmm.13567