Immortalized Human Trophoblast Cells - hTERT (Sw.71)

The Immortalized Human Trophoblast Cells - hTERT (Sw.71) were derived from 7-week first trimester placenta isolated from normal pregnancy and were virally infected with hTERT. The immortalized cells retained the same morphology as their primary counterparts. It has the abilities to spontaneously fuse as occasional multi-nucleated cells clusters may form, and can secrete cytokines (IL-1β, IL-6, IL-8, Monocyte Chemotactic Protein-1 (MCP-1), GRO (CXCR2), GRO-α (CXCL1), Intercellular Adhesion Molecule-1 (ICAM-1), Osteoprotegerin (OPG), Tissue Inhibitor of Metalloproteinase-1 (TIMP-1), TIMP-2 and VEGF). Cytokeratin 7 and vimentin markers are expressed. The population of cells is determined to be free of immune cells due to the absence of CD45 and CD68, and free of fibroblast cells due to the lack of fibroblast specific antigen. The Sw.71 cells are a valuable tool for trophoblast research.

Immortalization Method: Serial passaging and transduction with retroviruses expressing hTERT from the supernatant of pA317-hTERT cell line

BioSafety Level: II

Species: Human

Source Organ: Placenta

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: 14-24 hours

Seeding Density: 20,000-30,000 cells/cm²; Split ratio of 1:2 to 1:3

Markers: Cytokeratin 7 and Vimentin

Donor Age: 7-week old

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow IV medium available at abm (TM004). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10%, L-glutamine (G275) at a final concentration of 1%, 10mM HEPES (Sigma), 0.1 mM MEM non-essential amino acids (Sigma), 1 mM sodium pyruvate (TM057), and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1) hTERT activity was analyzed via TRAPeze ELISA Detection Kit; 2) Human Cytokine Array 3.1 and III was used to determine cytokine profile; 3) Immunocytochemistry was utilized for determining the presence of cell markers.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C