Immortalized Human Tracheal Smooth Muscle Cells

Human Primary Tracheal Smooth Muscles Cells are isolated from the trachea from single donors and stain positive for smooth muscle α-actin. They connect the endings of the tracheal cartilage by a contractile bridge called the paries membranaceus and consequently regulate the airway lumen. Under pathological conditions like asthma, the amount of smooth muscle cells in the trachea walls is increased leading to smooth muscle hypertrophy or hyperplasia. Hence, these cells are suitable for in vitro studies of asthma and other pulmonary diseases.

Immortalization Method: N/A

BioSafety Level: II

Species: Human

Source Organ: Lung

Growth Properties: Adherent

Morphology: Multipolar

Population Doubling: N/A

Seeding Density: 7,500 - 10,000 cells per cm²

Markers: smooth muscle alpha actin

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow X series medium available at abm, Cat. No. TM0494.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.

Quality Control: Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C