Immortalized Human Tonsillar Epithelial (HTE) cells are isolated from healthy adult patient biopsy and immortalized by being transfected with human papillomavirus type 16 (HPV16). HTE cells can be a useful cell line model as HPV-transfected human tonsillar epithelial cells for studying the HPV pathology and the role of HPV in the etiology of HPV related cancer. HTE cells can also be used as a cell model to study human tonsillar epithelial cell physiology and function.
Immortalization Method: HPV16 infection
BioSafety Level: II
Organism: Human (H. sapiens)
Species: Human
Source Organs: Oral
Organ Type: Oral
Growth Properties: Adherent
Morphology: Polygonal
Markers: E7 and E1^E4 expression, G418-resistant
Donor Age: Not disclosed
Propagation:Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The base medium for this cell line is Prigrow X medium available at abm, Cat. No. TM0721. Add 200 µg/ml G418 (G271) for selection pressure. Change media every 2-3 days.Carbon dioxide (CO2): 5%, Temperature: +37.0°C.
Quality Control: 1) Quantification of viral transcripts
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Spanos, W. C., Geiger, J., Anderson, M. E., Harris, G. F., Bossler, A. D., Smith, R. B., Klingelhutz, A. J., & Lee, J. H. (2008). Deletion of the PDZ motif of HPV16 E6 preventing immortalization and anchorage-independent growth in human tonsil epithelial cells. Head & neck, 30(2), 139–147. https://doi.org/10.1002/hed.20673Bienkowska-Haba, M., Luszczek, W., Myers, J. E., Keiffer, T. R., DiGiuseppe, S., Polk, P., Bodily, J. M., Scott, R. S., & Sapp, M. (2018). A new cell culture model to genetically dissect the complete human papillomavirus life cycle. PLoS pathogens, 14(3), e1006846. https://doi.org/10.1371/journal.ppat.1006846