Dermal fibroblasts are a dynamic and diverse population of cells whose functions in skin, in many respects, remain unknown. Fibroblasts engage in fibroblast-epidermal interactions during hair development and in interfollicular regions of skin and also play an important role in cutaneous wound repair and an ever-increasing role in bioengineering of skin. Skin equivalents can be constructed from immortalized keratinocytes and fibroblasts. In addition, it is ideal to establish feeder layers for various stem cell cultures and it can also be used as a model to study wound healing, toxicology or basic cell biology.Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying HPV E6/E7 geneBioSafety Level: IISpecies: HumanSource Organ: SkinGrowth Properties: AdherentMorphology: BipolarPopulation Doubling: N/ASeeding Density: N/AMarkers: Puromycin resistanceDonor Age: N/ADonor Gender: N/ADonor Ethnicity: N/APropagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, Penicillin/Streptomycin Solution (G255) to a final concentration of 1%, and L-glutamine (G275) to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: +37.0°C.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: Real Time PCR was used to quantify HPV E6 and E7 gene expression in immortalized cell line.Shipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C