Immortalized Human Retinal Pigment Epithelial Cells

Immortalized Human Retinal Pigment Epithelial Cells were isolated from neonatal human globes and spontaneously immortalized in culture. They retained typical morphology of the pigmented epithelium and expresses markers Chx10, Pax6, Ki67, and Oct4. It recommended for studies on the retinal pigment epithelium, and diseases associated such as macular degeneration and retinitis pigmentosa.

Immortalization Method: Spontaneous immortalization in culture

BioSafety Level: II

Species: Human

Source Organ: Retina

Growth Properties: Adherent

Morphology: Elongated with tapered ends

Population Doubling: 48 - 58 hours

Seeding Density: 30,000 - 50,000 cells/cm²

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: Grow cells in culture wares coated with the Gelatin Coating Solution (0.1%) (TM063) with the following conditions. The base medium for this cell line is Prigrow IV medium available from abm (TM004). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: Immunocytochemistry used to detect markers

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C