Immortalized Human Pulmonary Microvascular Endothelial Cells

The pulmonary microvascular endothelial cells (PMEC) are isolated from human pulmonary arteries. The PMECs form a semiselective barrier that is critical for lung gas exchange and regulation of fluid and solute passage between the blood and interstitial compartments in the lung. HPAEC have been used for the study of vascular permeability and inflammatory responses. HPAEC in co-culture with HPASMC have been used as a model for pulmonary angiopathy.

Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level: II

Species: Human

Source Organ: Lung

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: 25-35 hours

Seeding Density: 50,000 cells/cm²

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow I medium available at abm, Cat. No. TM001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C. * abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: Real Time PCR was used to quantify transgene expression.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C