SV40T integration site was noted to be on chromosome 1 (band 1p32)
Immortalization Method: Transfection with the plasmid pcc5, an SV40 construct containing the SV40 large T antigen gene linked to the SV40 promoter region
BioSafety Level: II
Organism: Human (H. sapiens)
Species: Human
Source Organs: Prostate
Organ Type: Prostate
Growth Properties: Adherent
Morphology: Epithelial-like
Donor Age: 63
Donor Gender: Male
Propagation: Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow II (TM002) + 10 ng/ml EGF + ITS Supplement (Collaborative Research) + 0.1 pM dexamethasone + 1% Penicillin/Streptomycin Solution (G255), +37.0°C, 5% CO₂
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Bae, V. L., Jackson-Cook, C. K., Brothman, A. R., Maygarden, S. J., & Ware, J. L. (1994). Tumorigenicity of SV40 T antigen immortalized human prostate epithelial cells: association with decreased epidermal growth factor receptor (EGFR) expression. International journal of cancer, 58(5), 721–729.Jackson-Cook, C.K., Bae, V.L., Edelman, W.,Brothman, A., Ware J. (1996). Cytogenetic Characterization of the Human ProstateCancer Cell Line P69SV40T and its Novel TumorigenicSublines M2182 and M15. Cancer Genetics, 87, 14-23Bae, V. L., Jackson-Cook, C. K., Maygarden, S. J., Plymate, S.R., Chen, J. & Ware, J. L. (1998). Metastatic Sublines of an SV40 Large T AntigenImmortalized Human Prostate Epithelial Cell Line. The Prostate, 34, 275-282.