Immortalized Human Periodontal Ligament Cells - hTERT

Description: Immortalized Human Periodontal Ligament Cells - hTERT are derived from human premolars. These cells were immortalized by transduction with recombinant lentivirus carrying hTERT gene. This cell line is an appropriate model for studying periodontal ligament regeneration.

Immortalization Method: Immortalized with lentivirus containing hTERT

BioSafety Level: II

Species: Human

Source Organ: Periodontal ligament

Growth Properties: Adherent

Morphology: N/A

Population Doubling: N/A

Seeding Density: N/A

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow VIII medium available at abm, Cat. No. TM018. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1) RT-PCR

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C