Immortalized Human Osteoprecursor Cells (OPC1)

The Immortalized Human Osteoprecursor Cells (OPC1) is a conditionally immortalized fetal osetoprecusor cell line uniquely expressing SV40 Tag in the presence of human interferon A/D which affects the proliferation capability. However, the Tag may be expressed constitutively in the presence of serum. It expresses alkaline phosphatase (ALP), osetocalcin (OC), osteonectin (ON), osteopontin (OP), procollagen-I (ProI), and PTH receptor (PTHR). The cells are able to differentiate in the presence of recombinant human BMP-2 (rhBMP2), a regulatory factor involved in differentiation. This cell line is valuable tool as a model system in studies in bone-graft substitutes.

Immortalization Method: Serial passaging and calcium phosphate-mediated transfection with a plasmid carrying SV40 T antigen, activated by human A/D interferon

BioSafety Level: II

Species: Human

Source Organ: Bone

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: 40-50 hours

Seeding Density: 2,800 cells/cm²

Markers: Alkaline phosphatase (ALP), osetocalcin (OC), osteonectin (ON), osteopontin (OP), procollagen-I (ProI), and PTH receptor (PTHR)

Donor Age: 12-13 weeks, fetal

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow VIII, available at abm, Cat. No. TM018>. To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 5%, 750 U/ml human A/D interferon (R&D Systems), Penicillin/Streptomycin Solution (G255) to a final concentration of 1%, and 0.2 mg/ml G418 for selection.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.
To differentiate add rhBMP2 at 10 ng/ml.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1) RT-PCR analysis for osteoblast markers; 2) ALP staining was utilized to determine presence of ALP

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C