Immortalized Human Oral Mucosal Epithelial Cells (hTERT-OME)

Primary oral epithelial cells were derived from patients undergoing removal of impacted teeth and were immortalized with the retroviral transfection of the pLXSN-hTERT vector to establish the Immortalized Human Oral Mucosal Epithelial Cells (hTERT-OME). The cells are also considered to be oral keratinocytes and have maintained their stem cell properties. hTERT-OME cells are a valuable tool for research in the biology of oral epithelial cells.

Immortalization Method: Transfection with the retroviral vector (pLXSN–hTERT)

BioSafety Level: II

Species: Human

Source Organ: Gingival mucosa

Growth Properties: Adherent

Morphology: Spindle shaped

Population Doubling: 25 - 35 hours

Seeding Density: 10,000 - 20,000 cells/cm²

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow IV medium available from abm (TM004). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10%, 200 µg/ml G418 (G271), and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C. * abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1) Immunocytochemistry showed the cells expressed cytokeratin and vimentin; 2) Western blot and RT-PCR detected the presence of hTERT

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C