Description: Primary oral epithelial cells were derived from patients undergoing removal of impacted teeth and were immortalized with the retroviral transfection of the pLXSN-hTERT vector to establish the Immortalized Human Oral Mucosal Epithelial Cells (hTERT-OME). The cells are also considered to be oral keratinocytes and have maintained their stem cell properties. hTERT-OME cells are a valuable tool for research in the biology of oral epithelial cells.Immortalization Method: Transfection with the retroviral vector (pLXSN–hTERT)BioSafety Level: IISpecies: HumanSource Organ: Gingival mucosaGrowth Properties: AdherentMorphology: Spindle shapedPopulation Doubling: 25 - 35 hoursSeeding Density: 10,000 - 20,000 cells/cm10²Markers: N/ADonor Age: N/ADonor Gender: N/ADonor Ethnicity: N/APropagation: The base medium for this cell line is Prigrow IV medium available from abm (TM004). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10%, 200 µg/ml G418 (G271), and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.
* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: 1) Immunocytochemistry showed the cells expressed cytokeratin and vimentin; 2) Western blot and RT-PCR detected the presence of hTERTShipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C