Nasal epithelial cells form the outermost protective layer against environmental factors. They clean, humidify, and warm inhaled air. They produces mucus, which binds particles that are subsequently transported to the pharynx by cilia on the epithelial cells. It has been shown that epithelial cells not only defend physically, but also respond by the production of inflammatory mediators that may affect local immune responses. They are used as an in vitro model for studying nasal defence mechanism. This cell line may contain some fibroblast-like cells, therefore exhibiting mixed morphology. Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigenBioSafety Level: IISpecies: HumanSource Organ: Nasal MucosaGrowth Properties: AdherentMorphology: PolygonalPopulation Doubling: 40 - 50 hoursSeeding Density: 10,000 - 15,000 cells per cm² (when thawing); recommended split ratio, post-thaw, of 1:2 (~25,000 cells/cm²)
These cells must be split at a low ratio (1:2) for successful growth.Markers: CytokeratinDonor Age: N/ADonor Gender: N/ADonor Ethnicity: N/APropagation: The base medium for this cell line is Prigrow X medium available at abm, Cat. No. TM0440.Carbon dioxide (CO2): 5%, Temperature: +37.0°C.
abm recommends using Accutase with T0440. This cell line is highly sensitive to trypsin.Quality Control: Real Time PCR was used to quantify SV40 gene expression in immortalized cell line. Tested negative for fungi and bacterial growth, mycoplasma, HIV-1, HBV, and HCV.Shipping Condition: Dry IceStorage Condition: liquid nitrogen or -180°C