Immortalized Human Mesothelial Cells - SV40

The Immortalized Human Primary Mesothelial cells-SV40 are derived from the human mesothelium, an important structure lining human abdomen cavities to provide lubrication, transportation and movement of fluids, immune cells, cytokines and proteins. These specialized cells are critical in regulating inflammatory responses and innate immune systems to protect against infections and tumor propagation. Their roles in cancer metastasis, metabolic diseases, peritoneal dialysis and surgical adhesions make them an ideal model for cancer research, pathological and/or therapeutic study.

Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level: II

Species: Human

Source Organ: Mesentery

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: 55 - 65 hours

Seeding Density: 5,000 - 15,000 cells/cm²

Markers: APOBEC3, CD105, VEGF

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow I medium available at abm, Cat. No. TM001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, Penicillin/Streptomycin Solution (G255) to a final concentration of 1%, and 50 ng/ml hEGF (Z100135).
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C. * abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C