Immortalized Human Lens Epithelial Cells - SV40

The Immortalized Lens Epithelial Cells - SV40 is established from primary cells (isolated from infant lens epithelial fragments) using Adl2-SV40 adenovirus. The Immortalized Human Lens Epithelial Cells - SV40 provides a homogeneous population of cell for the investigation of the physiology of the normal human lens epithelium and study its response to cataractogenic agents and the human lens specific functions. These cells also provide a unique system for examining the relationship between gene expression and differentiation.

Immortalization Method: Serial passaging and transduction with recombinant adenovirus carrying SV40 Large T antigen

BioSafety Level: II

Species: Human

Source Organ: Infant lens

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: 76 hours

Seeding Density: 5,000 - 10,000 cells/cm²; Recommended split ratio is 1:2

Markers: None

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C. * abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: The Immortalized Human Lens Epithelial Cells react positively with the monoclonal antibody to SV40 large T antigen. Western blot and precipitation are used to confirm the presence of βH- and γ-crystallin, the proteins that are markers for cell differentiation in the lens epithelium. SV40 was observed through IHC (Figure 6).

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C