Immortalized Human Laryngeal Posterior Commissure Cells

The posterior commissure region is made up of cells which aid in vocalization. More importantly, it is the region of the larynx most associated with the pharynx. This makes it the first area to be exposed to material from the stomach during laryngopharyngeal reflux (LPR); a mechanism suggested contributing to cancers and anomalies in the larynx. Due to its contact with LPR, posterior commissure cells are an attractive model to study laryngeal disorders. The Immortalized Laryngeal Posterior Commissure Cell Line retains its squamous phenotype and continues to express CK5 and CK8 markers after immortalization.

Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying HPV E6/E7 gene

BioSafety Level: II

Species: Human

Source Organ: Larynx

Growth Properties: Adherent

Morphology: Epithelial (<10% fibroblasts)

Population Doubling: N/A

Seeding Density: 20,000 - 30,000 cells per cm².

Markers: CK5, CK8

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The medium for this cell line is Prigrow X.1 medium available at abm, Cat. No. TM010.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.

Quality Control: Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C