Description: Immortalized Human Intervertebral Disc Cartilage Endplate-derived Stem Cells -SV40 is a valuable and reliable source for studies relating to the cartilaginous endplate and nutrient transportation in the body. The cartilage is positioned between the vertebral endplate in the spine and the nucleus pulposus in which it functions as a barrier. It has a role in the health aspects of the disc such as growth and degeneration. With an immortalized cell line, researchers do not need to constantly look for donors to isolate the cells. These cells can be proliferated in vitro for downstream experiments.
Immortalization Method: Infected with a non-replicating SV40 lentivirus
BioSafety Level: II
Species: Human
Source Organ: Cartilaginous endplate
Growth Properties: Adherent
Morphology: Polygonal
Population Doubling: N/A
Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers: N/A
Donor Age: N/A
Donor Gender: N/A
Donor Ethnicity: N/A
Propagation: The base medium for this cell line is Prigrow IV medium available at abm (TM004). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.
* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C