Immortalized Human Hepatocytes - HPV E6/E7, hTERT & MycT58A

Description: Hepatocytes are the chief functional cells of the liver and perform an astonishing number of metabolic, endocrine, and secretory functions. They are cuboidal epithelial cells, and 70–85% of the liver volume is occupied by hepatocytes.
Human hepatocytes are routinely used by the pharmaceutical industry for study of hepatotoxicity, drug clearance, and drug-drug interactions. However, their use is largely impeded by inadequate supply, high cost, high variability, and limited in vitro proliferation capacity. Alternative cell sources include hepatic cell lines and stem-cell derived hepatocytes but most of them do not exhibit sufficient in vivo-like functionality to be of pharmaco-toxicological relevance. In this scenario, immortalized human hepatocytes serve as the optimal in vitro model of the human liver as they are more easily maintained, propagated in culture, and have less intrinsic variation. They provide a valuable tool for investigating metabolic pathways and hepatocyte proliferation-related events.

Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying HPV E6/E7, hTERT & MycT58A genes

BioSafety Level: II

Species: Human

Source Organ: Liver

Growth Properties: Adherent

Morphology: Epithelial

Population Doubling: 30 - 35 hours

Seeding Density: 30,000 cells/cm10²; Recommended split ratio of 1:2 to 1:5*
*Do not exeed a split ratio of 1:5

Markers: AAT1, CK8, CK18 and CYP1A1

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation:Grow cells in culture vessels coated with Collagen I, rat tail. Coat plates at +37.0°C overnight and wash with sterile PBS prior to use.
The base media for this cell line is Prigrow IX medium available at abm Cat. No. TM019. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.
* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: N/A

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C