Immortalized Human Hepatic Sinusoidal Endothelial Cells-SV40

Hepatic Sinusoidal endothelial cells are microvascular endothelial cells with a phenotype resembling dendritic cells and a unique function as antigen-presenting cells for CD4+ T cells. These specialized cells are dynamic regulators of porosity responding rapidly to local environmental zonal stimuli during liver regeneration. Importantly, naive CD4+ T cells primed by antigen-presenting LSEC differentiate into regulatory T cells. Thus, LSEC represent a new type of organ-resident "non-professional" antigen-presenting cell that appears to be involved in the local control of the immune response and the induction of immune tolerance in the liver.

Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level: II

Species: Human

Source Organ: Liver

Growth Properties: Adherent

Morphology: Polygonal

Population Doubling: 12-22 hours

Seeding Density: 20, 000 cells/cm²; Recommended split ratio is 1:3 to 1:4

Markers: N/A

Donor Age: Adult

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow I medium available from abm (TM001). To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 5%, Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C. * abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C