Immortalized Human Hair Follicle Dermal Papilla Cells –SV40T/c-Myc

Human Hair Follicle Dermal Papilla cells are highly active mesenchymal cells isolated from the hair papilla embedded in extracellular matrix of scalp hair follicles. Dermal Papilla Cells play a significant role in controlling the hair growth cycle and production by involving in the epithelial-mesenchymal interaction of hair follicle cells. The Immortalized Human Dermal Papilla Cells (DPCs) express DPC specific markers smooth muscle alpha actin and alkaline phosphatase and retain the ability to secrete growth factors such as VEGF, PIGF, bFGF, IGFBP-6 and GM-CSF. DCP-conditioned media have also been shown to increase proliferation of HaCaT cells in vitro and to accelerate hair growth in mice models. A list of up-regulated genes in the DPC can be found in the supporting document section below. The DPC serves as a useful tool for the study of hair growth regulation.

Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen and c-Myc gene

BioSafety Level: II

Species: Human

Source Organ: Hair follicles

Growth Properties: Adherent

Morphology: Flat|Polygonal

Population Doubling: N/A

Seeding Density: 20,000 - 30,000 cells/cm², Recommended Split ratio of 1:2

Markers: smooth muscle alpha actin, alkaline phosphatase

Donor Age: Donor Info Not Disclosed

Donor Gender: Donor Info Not Disclosed

Donor Ethnicity: Donor Info Not Disclosed

Propagation: The base medium for this cell line is Prigrow III medium available from abm (TM003). To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and 1% Penicillin/streptomycin.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: 1) Western blot was used to identify SV40T and c-myc transgene expression. 2) Immunocytochemistry and western blot were performed to assess the expression of smooth muscle actin alpha and alkaline phosphatase. 3) Up-regulated genes in DPCs is analyzed by DAVID (the Database for Annotation, Visualization and Integrated Discovery).

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C