Human fetal hematopoietic cells were immortalized by stable lentiviral (LTRCMVR2) overexpression of human telomerase reverse transcriptase (hTERT). Resulting cells were subcloned and continued to proliferate in culture to establish the Immortalized Human Fetal Liver Cells (cBAL111). The cells are positive for hepatic markers albumin, glutamin synthetase, cytokeratin 18, and for the cholangiocyte marker cytokeratin 19.
Immortalization Method: Immortalized with lentivirus containing hTERT
BioSafety Level: II
Organism: Human
Species: Human
Source Organs: Liver
Organ Type: Liver
Growth Properties: Adherent
Morphology: Spindle-shaped
Population Doubling: 12 days; have contact inhibition
Seeding Density: 300,000 cells/cm
2Markers: Ampicillin & neomycine-resistant, expresses albumin, glutamin synthetase, cytokeratin 18, and cytokeratin 19.
Donor Age: 14 - 18 weeks
Propagation: The base medium for this cell line is Prigrow III medium available at
abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum (TM999)* to a final concentration of 10%, 1X L-glutamine (G275), 1 µM dexamethason (Sigma), 10 µg/mL insulin, 5.5 µg/mL transferrin, 6.7 ng/mL selenium-X (ITS mix, Life Technology), and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Change media every 2-3 days.Carbon dioxide (CO
2): 5%, Temperature: +37.0°C.
Quality Control: 1. qRT-PCR for hTERT expression. 2. cBAL111 clone continued to proliferate whereas primary cells senescenced in culture. 3. Immunostaining conducted showed cells are positive for hepatic markers albumin, glutamin synthetase and cytokeratin 18, and positive for cholangiocyte marker cytokeratin 19.
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Deurholt, T., ten Bloemendaal, L., Chhatta, A. A., van Wijk, A. C., Weijer, K., Seppen, J., Elferink, R. P., Chamuleau, R. A., & Hoekstra, R. (2006).
in vitro functionality of human fetal liver cells and clonal derivatives under proliferative conditions. Cell transplantation, 15(8-9), 811–822. https://doi.org/10.3727/000000006783464417Poyck, P. P., van Wijk, A. C., van der Hoeven, T. V., de Waart, D. R., Chamuleau, R. A., van Gulik, T. M., Oude Elferink, R. P., & Hoekstra, R. (2008). Evaluation of a new immortalized human fetal liver cell line (cBAL111) for application in bioartificial liver. Journal of hepatology, 48(2), 266–275. https://doi.org/10.1016/j.jhep.2007.09.018Norton, P., Comunale, M. A., Herrera, H., Wang, M., Houser, J., Wimmerova, M., Romano, P. R., & Mehta, A. (2016). Development and application of a novel recombinant Aleuria aurantia lectin with enhanced core fucose binding for identification of glycoprotein biomarkers of hepatocellular carcinoma. Proteomics, 16(24), 3126–3136. https://doi.org/10.1002/pmic.201600064