Cells may undergo neural differentiation (see Goorha S & Reiter LT. Curr Protoc Hum Genet. 2017 Jan 11;92:21.6.1-21.6.10.) in which neural cells expresses TUJ1, NeuN and GFAP
Immortalization Method: Infected with pBABE-hTERT-Puro retrovirus
BioSafety Level: II
Organism: Human (H. sapiens)
Species: Human
Source Organs: Oral
Organ Type: Oral
Growth Properties: Adherent
Morphology: Fibroblast
Seeding Density: 20,000 cells/cm²; Recommended split ratio is 1:3
Donor Age: Donor Info Not Disclosed
Donor Gender: Donor Info Not Disclosed
Propagation: Use of Poly-D-Lysine is required for cell adhesion to the culture vessels. PriGrow IV (TM004) + 20% FBS + 1% Penicillin/Streptomycin Solution (G255), +37.0°C, 5% CO₂
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Urraca, N., Memon, R., El-Iyachi, I., Goorha, S., Valdez, C., Tran, Q. T., Scroggs, R., Miranda-Carboni, G. A., Donaldson, M., Bridges, D., & Reiter, L. T. (2015). Characterization of neurons from immortalized dental pulp stem cells for the study of neurogenetic disorders. Stem cell research, 15(3), 722–730. https://doi.org/10.1016/j.scr.2015.11.004Wilson, R., Urraca, N., Skobowiat, C., Hope, K. A., Miravalle, L., Chamberlin, R., Donaldson, M., Seagroves, T. N., & Reiter, L. T. (2015). Assessment of the Tumorigenic Potential of Spontaneously Immortalized and hTERT-Immortalized Cultured Dental Pulp Stem Cells. Stem cells translational medicine, 4(8), 905–912. https://doi.org/10.5966/sctm.2014-0196Goorha, S., & Reiter, L. T. (2017). Culturing and Neuronal Differentiation of Human Dental Pulp Stem Cells. Current protocols in human genetics, 92, 21.6.1–21.6.10. https://doi.org/10.1002/cphg.28