This cell line was generated using primary SuPs derived from patients with craniosynostosis, with retroviruses carrying SV40 T antigen, flanked by FRT or loxP sites, using hygromycin B as a selection marker. These cells continuously proliferate in culture, express a large number of mesenchymal stem cell markers, and retain osteogenic and adipogenic potential upon addition of BMP9 to the media. The immortalization can be reversed by expression of FLP recombinase to excise the SV40 T gene. iSuPs are a valuable tool for studying craniosynostosis, intramembranous ossification, and adipogenesis, as well as potential therapeutic drugs for associated pathologies.
Immortalization Method: Immortalized with retrovirus containing SV40 T antigen, flanked by FRT or loxP sites, selected with hygromycin B
BioSafety Level: II
Organism: Human
Species: Human
Source Organs: Brain
Organ Type: Brain
Growth Properties: Adherent
Morphology: Fibroblast-like
Markers: CD73, CD105 (endoglin), CD90 (Thy-1), CD166 (ALCAM), BMPR-II, CD117 (c-kit), CD29 (Integrin β1)
Donor Age: 15-17 months
Donor Gender: Male
Propagation: The base medium for this cell line is Prigrow III medium available at
abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Change media every 2-3 days.Carbon dioxide (CO
2): 5%, Temperature: +37.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Song, D., Zhang, F., Reid, R. R., Ye, J., Wei, Q., Liao, J., Zou, Y., Fan, J., Ma, C., Hu, X., Qu, X., Chen, L., Li, L., Yu, Y., Yu, X., Zhang, Z., Zhao, C., Zeng, Z., Zhang, R., Yan, S., … He, T. C. (2017). BMP9 induces osteogenesis and adipogenesis in the immortalized human cranial suture progenitors from the patent sutures of craniosynostosis patients. Journal of cellular and molecular medicine, 21(11), 2782–2795. https://doi.org/10.1111/jcmm.13193