Immortalization Method: Serial passaging and transduction with recombinant VSV-G retrovirus carrying hTERT and GFP genes
BioSafety Level: II
Species: Human
Source Organ: Bone Marrow
Growth Properties: Adherent
Morphology: Spindle shaped
Population Doubling: 35-45 hours
Seeding Density: 10,000 - 20,000 cells/cm²; Recommended split ratio is 1:2 to 1:3
Markers: CD44 and CD73. These cells also express markers of osteoblasts (alkaline phosphatise) and chondrocytes (incorporation of toluidine blue dye)
Donor Age: N/A
Donor Gender: N/A
Donor Ethnicity: N/A
Propagation: The base medium for this cell line is Prigrow II medium available at abm, Cat. No. TM002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, hydrocortisone to 10-6 mol/L and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.Quality Control: 1. Immunofluorescence and RT-PCR were used to confirm the presence hTERT expression. 2. PCR based TRAP was used to assay telomerase activity. 3. Neo concentration not determined for selection use but cells were sorted for GFP+ populations
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C