Immortalized Hair Follicle Dermal Papilla Cells - SV40

Human Hair Follicle Dermal Papilla cells are highly active mesenchymal cells isolated from the hair papilla embedded in extracellular matrix of scalp hair follicles. Dermal Papilla Cells play a significant role in controlling the hair growth cycle and production by involving in the epithelial-mesenchymal interaction of hair follicle cells. Their survival is regulated by signal transduction pathways such as ERK and Akt. These cells can be used for development and evaluation of hair growth products and identifying cell populations within the hair follicle. In addition, they can be used for in vitro screening of androgen blocking reagents since they have androgen receptors.

Immortalization Method: Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level: II

Species: Human

Source Organ: Hair Follicle

Growth Properties: Adherent

Morphology: Multipolar

Population Doubling: 40-42 hours

Seeding Density: 6,000 - 10,000 cells per cm²

Markers: N/A

Donor Age: 56

Donor Gender: Female

Donor Ethnicity: Caucasian

Propagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C. * abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C