The parental L6 myoblast cell line has been transfected with GLUT4 cDNA to give the Immortalized Rat Skeletal Myoblast Cell line (L6). The cells display a myoblast morphology and retain the potential to differentiate. In the plasma membrane, GLU4 is responsible for the activation of glucose transportation by insulin. The Immortalized Rat Skeletal Myoblast Cell line (L6) has been used to study glucose transport systems and is suitable for research pertaining to muscle physiology and metabolism.
Immortalization Method: Immortalized by treatment with 3-methylcholanthrene
BioSafety Level: II
Species: Rat
Source Organ: Skeletal muscle
Growth Properties: Adherent
Morphology: Polygonal
Population Doubling: 20-30 hours
Seeding Density: 20,000-30,000 cells/cm²; Recommended split ratio of 1:5 to 1:10
Markers: Blasticidin-HCl resistance
Donor Age: N/A
Donor Gender: N/A
Donor Ethnicity: N/A
Propagation: The base medium for this cell line is Prigrow VIII medium available at abm, Cat. No. TM018. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C. The differentiation media is Prigrow VIII, 2% FBS (TM999)*, and Antibiotic/Antimycotic (100X) (G273). Cells will reach confluence after 2-3 days in culture using the recommended seeding density. After cells are confluent, switch cultures from growth media to differentiation media, then culture for another 2-3 days. Use myotubles on the 7th day after seeding, as myotubles may begin to lift off the plate if cultured for too long. Avoid culturing at low densities, as the L6 muscle myoblasts cells have the tendency to grow in tightly packed patches called islets. Change media every 2-3 days.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control: N/A
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C