Immortalized Feline Aortic Endothelial Cells - SV40 & hTERT

Immortalized Feline Aorta Endothelial Cells - SV40 & hTERT are derived from primary aortic endothelial cells isolated from cats. The endothelium is involved in a variety of pathological processes, such as tumor invasion, atherosclerosis, arthritis, thrombosis, and vasculitis. With a feline model, scientists may use this model to further expand the knowledge in endothelial-related diseases in feline or can use this model to address to diseases in humans as well. The Immortalized Feline Aorta Endothelial Cells - SV40 & hTERT is a valuable source for long-term studies as the cells can be continuously cultured for experiments, and retains both the phenotype and characteristics as primary cells.

Immortalization Method: Co-transduced with non-replicating lentivirus carrying SV40LT and hTERT

BioSafety Level: II

Species: Feline

Source Organ: Aorta

Growth Properties: Adherent

Morphology: Elongated

Population Doubling: N/A

Seeding Density: Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available at abm (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to final concentration of 10%, 0.1 mg/ml gentamycin, 1 mM sodium pyruvate (TM057), 1% non-essential amino acids(TM068), 50 ?g/ml endothelial cell growth supplement (ECGS; Biomedical Technologies Inc.), and 10 U/ml heparin (Sigma; H5515) and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: Immunodetection for hTERT and SV40L T antigen

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C