Immortalized Canine Dermal Fibroblast - EBV

Immortalization Method: Serial passaging and infection with Epstein-Barr Virus (EBV)

BioSafety Level: II

Species: Canine

Source Organ: Skin

Growth Properties: Adherent

Morphology: Bipolar

Population Doubling: 50 - 60 hours

Seeding Density: 20,000 – 50,000 cells/cm²

Markers: N/A

Donor Age: N/A

Donor Gender: N/A

Donor Ethnicity: N/A

Propagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: +37.0°C. * abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control: N/A

Shipping Condition: Dry Ice

Storage Condition: liquid nitrogen or -180°C