Immortalization Method: Transfected with the gene for TERT, cloned to pure populations by limited dilution culture and were expanded
BioSafety Level: II
Organism: Human (H. sapiens)
Species: Human
Growth Properties: Adherent
Morphology: Polygonal
Population Doubling: 30 hours
Seeding Density: 25,000-30,000 cells/cm^2
Markers: Absence of AT1 specific markers, able to bind SARS-CoV-2 spike protein
Propagation: Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow X Series Medium (TM0825), +37.0°C, 5% CO₂
Quality Control: Cells were characterized by IFA confocal microscopy
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C