Conditionally Immortalized Stria Vascularis Cell Line (SV-k1) was derived from transgenic mouse which constitutively expresses a temperature-sensitive simian virus 40 T antigen (tsA58). The cells proliferate at +33.0°C, and lack the expression of prestin and the voltage dependent potassium channel (I
sk), but express occulin. These cells are a valuable model for strial capillary basement membrane studies.
Immortalization Method: Derived from transgenic mouse which constitutively expresses a temperature-sensitive simian virus 40 T antigen (tsA58).
BioSafety Level: II
Organism: Mouse
Species: Mouse
Source Organs: Stria vascularis
Organ Type: Stria vascularis
Growth Properties: Adherent
Morphology: Epithelial
Markers: Prestin (-), occulin (+)
Propagation: The base medium for this cell line is Prigrow III medium available at
abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Change media every 2-3 days.Carbon dioxide (CO
2): 10%, Temperature: +33.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.To differentiate cells: after growing cells to 80% confluence at +33.0°C, move cells to 39.0°C and 5% CO
2 conditions for 2-3 weeks. Cells will slowly stop growing and will die off, with all cells dying after approximately 4 weeks.
Shipping Condition: Dry Ice
Storage Condition: liquid nitrogen or -180°C
Reference: Espreafico, E. M., Coling, D. E., Tsakraklides, V., Krogh, K., Wolenski, J. S., Kalinec, G., & Kachar, B. (1998). Localization of myosin-V in the centrosome. Proceedings of the National Academy of Sciences of the United States of America, 95(15), 8636–8641. https://doi.org/10.1073/pnas.95.15.8636Gratton, M. A., Meehan, D. T., Smyth, B. J., & Cosgrove, D. (2002). Strial marginal cells play a role in basement membrane homeostasis:
in vitro and
in vivo evidence. Hearing Research, 163(1-2), 27-36. doi:10.1016/s0378-5955(01)00358-6Kalinec, G. M., Park, C., Thein, P., & Kalinec, F. (2016). Working with Auditory HEI-OC1 Cells. Journal of visualized experiments : JoVE, (115), 54425. https://doi.org/10.3791/54425Park, C., Thein, P., Kalinec, G., & Kalinec, F. (2016). HEI-OC1 cells as a model for investigating prestin function. Hearing Research, 335, 9-17. doi:10.1016/j.heares.2016.02.001