PSMA Stably Expressing U87MG/ΔEGFR (PSMA/FOLh1/Carboxypeptidase II) GFP Cell Line

Folate hydrolase 1 (FOH1 or PSMA) encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. This protein is up-regulated in prostate cancer cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. Thus, this cell line could be helpful in the field of prostate cancer drug discovery.

Quantity: 10⁶ cells/1.0 ml

Organism: Human

Source Organ: Brain

BioSafety Level: II

Growth Properties: Adherent

Morphology: Epithelial-like

Population Doubling: 30-40 hours

Markers: Zeocin

Applications: For Research Use Only

Propagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the completed growth medium, add the following components to the base medium: heat inactivated fetal bovine serum to a final concentration of 10% , and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%, and 150?g/ml zeocin . Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.

Freeze Thaw: 1. Pre-warm complete media in a +37°C waterbath.2. Remove the cryopreserved vial from the liquid nitrogen storage tank.3. Thaw the cells quickly by placing the lower half of the vial into the +37°C water bath and remove after 60 seconds. There should still be a few ice crystals left after thawing. It is important not to over-thaw the cryovials as the presence of DMSO is toxic to the cells.4. Re-suspend the cells in the vial and transfer the cell suspension into a 15 ml sterile conical tube containing 5 ml complete media.5. Centrifuge cells at 1500rpm for 3 minutes to pellet.6. Aspirate out the media, leaving cell pellet undisturbed.7. Re-suspend pellet in fresh culture medium and plate in new culture vessel.8. Incubate cultures at +37°C, 5% CO2.

Quality Control: 1. Real Time PCR was used to quantify PSMA gene expression in immortalized cell line. 2. Puromycin selection. 2. Puromycin selection

Shipping Conditions: Dry Ice

Storage Conditions: -180°C