Luciferase is commonly used as a reporter to assess the transcriptional activity of a genetic construct with luciferase gene under the promoter of interest. NF-kB Luciferase Stable RAW264.7 Cell Line has been stably transfected with a 3kB-Luc SV40 reporter, which contains 3 NF-kB sites from the interferon gene upstream of the luciferase coding region. This cell line is useful in investigation of NF-kB activation in monocyte-macrophage models. This cell line should be maintained in 100 µg/ml G418. Quantity: 10⁶ cells/1.0 mlOrganism: MouseSource Organ: AscitesBioSafety Level: IIGrowth Properties: AdherentMorphology: MacrophagePopulation Doubling: 30-35 hoursSeeding Density: 50,000-70,000 cells/cm2. Recommended split ratio of 1:5Applications: For Research Use OnlyPropagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10%, L-Glutamine to 2mM, 100 µg/ml G418, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: +37.0°C. Quality Control: Have been tested for luciferase activityShipping Conditions: Dry IceStorage Conditions: -180°C