Catalog Number: ABMT3006
Name: Luciferase Stable PC3 Cell Line
Description: Luciferase is commonly used as a reporter to assess the transcriptional activity of a genetic construct with luciferase gene under the promoter of interest. The Luciferase stable PC3 Cell line was generated using abm's <a href="https://www.abmgood.com/luciferase-renilla-reniformis-lentiviral-vector-cmv-2.html#LV010087#TM004">LV010087</a> on PC3 cells. The expression of luciferase has been confirmed with luciferase assay. All clones are puromycin (0.1 µg/ml)-resistant. Luciferase stable PC3 cells can be a very useful cell line for non-invasive visualization in in vitro experiments.
Organism: Human (H. sapiens)
Tissue: Prostate
Donor History: Male, 62, Caucasian, Prostate carcinoma
Growth Properties: Adherent, epithelial-like
Cell Type: Stable Cell Lines
Storage Condition: Vapor phase of liquid nitrogen, or below -130°C.
Shipping Conditions: Ship with dry ice.
Product Format: Frozen
Intended Use: This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
BioSafety: II
Growth Conditions: Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow IV (TM004) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255), +37.0°C, 5% CO₂. Selection with 0.1 µg/ml Puromycin (G264)
Unpacking and Storage Instructions:
1. Visually examine the packaging containers for signs of leakage or breakage.
2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130°C, preferably in liquid nitrogen vapor phase storage, until ready for use.
To ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130°C or in liquid nitrogen vapor phase. Do not store at -70°C, as it will result in loss of viability.
Subculture Protocol: Volumes given below are for a T75 flask; proportionally increase or decrease the volume as required per culture vessel size. Subculture cells once the culture vessel is 80% confluent.
1. Aspirate the culture media, and add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel.
2. Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in +37°C, for several minutes to facilitate detachment.
3. Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel.
4. Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.
5. Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired.
6. Incubate the cells at the recommended conditions.
Cryopreservation: Cryopreservation Medium (TM024), or complete growth media with 10% DMSO.
Split Ratio: 1:2 or 1:3
Seeding Density (cells/cm2): 40,000-60,000
Population Doubling Time (h): 45-52
Application: Research Use Only.