Luciferase is a class of oxidative enzymes that produce bioluminescence, when expressed in cells they are used for microscopy and as a reporter gene. 17L3C cells were derived from a mammary tumor in a female MMTV-PyVT transgenic mouse (FVB/n strain). Luciferase Stable 17L3C (17L3C-luc) Cell Line presents a model which allows real-time observations of tumor growth in vivo .Quantity: 10⁶ cells/1.0 mlOrganism: Mouse (M. musculus)Source Organ: BreastBioSafety Level: IIGrowth Properties: AdherentMorphology: EpithelialMarkers: Luciferase, Puromycin resistance Applications: For Research Use OnlyPropagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum (TM999) to a final concentration of 10%, 50 µg/ml gentamicin (G272), and 5 μg /ml Puromycin (G264). Change media every 2-3 days.Carbon dioxide (CO2): 5%, Temperature: +37.0°C.Preservation: 1. Freeze Medium: Complete growth medium with 10% FBS and 10% DMSO.2. Storage Temperature: Liquid nitrogen vapour phase.Quality Control: 1) Bioluminescent imagingShipping Conditions: Dry IceStorage Conditions: -180°C