GFP-Tagged Tctex-type 3 Stable Expressing HeLa Cell Line

Dynein is a group of motor proteins in the cytoskeleton. TcTex is a component of the dynein 1 protein complex that helps dynein to link to cargo and helps the protein function to transport vesicles and organelles along the cytoskeleton. Correct separation of chromosomes during mitosis is mediated by kinetochores and components of the cytoskeleton and this process employs many interacting protein complexes. The GFP-Tagged Tctex-type 3 Stable Expressing HeLa Cell Line has previously been used in studies to investigate kinetochores movement during mitosis and the function of various proteins involved in this process.High magnifications (400-1000x) are required to observe the GFP-tagged fusion due to the intracellular localization of specific subcellular structures.

Quantity: 10⁶ cells/1.0 ml

Organism: Human

Source Organ: Cervix

BioSafety Level: II

Growth Properties: Adherent

Morphology: Epithelial

Population Doubling: 24-34 hours

Seeding Density: 40,000-50,000 cells/cm²

Markers: GFP

Applications: For Research Use Only

Propagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, Penicillin/Streptomycin Solution (>G255) to a final concentration of 1%, and L-glutamine (G275) to a final concentration of 1%. Change media every 2-3 days.Carbon dioxide (CO₂): 5%, Temperature: +37.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Preservation: 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.2. Storage Temperature: Liquid nitrogen vapour phase.

Shipping Conditions: Dry Ice

Storage Conditions: -180°C