These particular HeLa cells stably express the LIS1 protein. which is also known as Platelet-activating factor acetylhydrolase IB subunit alpha (PAFAH1B1). It is part of a complex called platelet activating factor acetylhydrolase 1B (PAFAH1B). PAFAH1B controls the amount of platelet activating factor (PAF) in the brain, which is thought to control neuronal movement. PAFAH1B1 also interacts with microtubules and is thought to be involved in cytoskeleton organization. PAFAH1B1 is one of the proteins that when the gene encoding it is lost or mutated, results in Miller–Dieker syndrome.High magnifications (400-1000x) are required to observe the GFP-tagged fusion due to the intracellular localization of specific subcellular structures.
Quantity: 1million cells/1.0 ml
Organism: Human (H. sapiens)
Source Organ: Cervix
BioSafety Level: II
Growth Properties: Adherent
Morphology: Epithelial
Applications: For Research Use Only
Propagation: The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, 2 mM L-glutamine (G275) and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.Change media every 2-3 days.Carbon dioxide (CO2): 5%, Temperature: +37.0°C.* Do not use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control: 1) Phenotypic analyses via microscopy
Shipping Conditions: Dry Ice
Storage Conditions: -180°C