AIZ-AR Stable 22Rv1 Luciferase Reporter Cell Line

The androgen receptor (AR) is a ligand-activated transcription factor involved in developmental and psychological processes and implicated in the pathogenesis of prostatic hyperplasia, prostate cancer, and altered pubertal development. The AIZ-AR Stable 22Rv1 Luciferase Reporter Cell Line contains a reporter plasmid with three copies of the androgen response region with a downstream androgen response element which allows for the detection of AR ligands through the induction of luciferase activity as early as 8 hours after treatment. The reporter plasmid p3ARR/ARE-luc2P/minP/hygro was successfully transfected into the 22Rv1 cells using the Fugene HD transfection reagent. These immortalized cells are functional for over 25 passages after cryopreservation making them a reproducible and sensitive tool for the detection of AR ligands during research investigating novel pharmaceutical drugs or screening for environmental pollutants that may disrupt endocrine function through AR interaction.

Quantity: 10⁶ cells/1.0 ml

Organism: Human

Source Organ: Prostate

BioSafety Level: II

Growth Properties: Adherent

Morphology: Epithelial-like

Population Doubling: 49 - 59 hours

Seeding Density: 40,000 cells/cm² to 80,000 cells/cm²

Applications: For Research Use Only

Propagation: The base medium for this cell line is Prigrow II medium available at abm, Cat. No. TM002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10%, 2 mM L-glutamine (G275), 1 mM Sodium Pyruvate Solution (TM057) and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Add 0.2 mg/ml of Hygromycin B (G265) once cells recover from thawing. Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Split ratio: 1:3 or 1:5.

Please note these cells are slow growing in culture. Media should be changed every 2-3 days. Must use 0.5% Trypsin-EDTA (TM051 or TM050) for subculturing of the cells.

Quality Control: Selective growth on media containing hygromycin B

Shipping Conditions: Dry Ice

Storage Conditions: -180°C