The spleen stores and purifies erythrocytes, metabolizes hemoglobin, and recycles iron. The spleen also provides a critical function for the immune system by mounting a primary immune response to antigens in the blood and synthesizing antibodies [1]. Splenocytes are mononuclear cells derived from the spleen and include T-lymphocytes, B-lymphocytes, NK-cells, and NK Tcells. Primary rat splenocytes (RS) can be used to isolate CD4+ T-cells, CD8+ T-cells, and CD45R+ B cells.
Recommended Medium
It is recommended to use HematoGro Medium (Cat. No. SC5501) for culturing in vitro.
Product Use
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice
Warranty
Cells are only warranted if ScienCell media and reagents are used and the recommended protocols are followed.
References
[1] Mebius R, Kraal G. (2005) "Structure and function of the spleen." Nat Rev Immunol 5(8): 606-616.