These cells are involved in the production and maintenance of the basement membrane in the seminiferous tubules [1]. TPC also contributes to spermatogonial stem cell maintenance through the secretion of niche factors [1]. Disruption to peritubular cell development result in aberrations in reproductive development and physiology [2, 3]. Specifically, alteration in the smooth-muscle like phenotype of TPC is one of the phenotypes associated with impaired spermatogenesis [3].
Recommended Medium: It is recommended to use Smooth Muscle Cell Medium (SMCM, Cat. no. SC1101) for culturing RTPC in vitro.
Product Use: RTPC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping: Dry ice.
References: [1] Tao HP, Lu TF, Li S, Jia GX, Zhang XN, Yang QE. (2023) “Pancreatic lipase-related protein 2 is selectively expressed by peritubular myoid cells in the murine testis and sustains long-term spermatogenesis.” Cell Mol Life Sci 80: 217.
[2] Garza S, Papadopoulos. (2023) “Cops5 in Peritubular Myoid Cells influences Reproductive Development and Hormone Production.” Endocrinology. 164 (7): bqad092.
[3] Mayerhofer A, Walenta L, Mayer C, Eubler K, Welter H. (2018) “Human testicular peritubular cells, mast cells and testicular inflammation.” Andrologia. 50 (11): e13055.