Renal mesangial cells are perivascular cells located within the central portion of the glomerular tuft between capillary loops, constituting 30-40% of the total glomerular cell population [1]. They regulate the intraglomerular capillary flow and ultrafiltration surface via mesangial cell contraction and release of growth factors and vasoactive agents [2]. By pinocytosis and phagocytosis, mesangial cells remove local accumulation of macromolecules in the mesangial space [3]. Mesangial cells also synthesize, assemble and control turnover of the mesangial matrix. Overproduction of mesangial cells has been observed in various glomerular diseases, such as IgA nephropathy, mesangioproliferative glomerulonephritis, lupus nephritis, glomerulosclerosis and diabetic nephropathy [4]. Such critical involvements suggest renal mesangial cells are an ideal model for studying mesangial injury and glomerular functions under both physiological and pathophysiological conditions.
Recommended Medium
It is recommended to use Mesangial Cell Medium (MCM, Cat. No. SC4201) for culturing RRMC in vitro.
Product Use
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice
Warranty
Cells are only warranted if ScienCell media and reagents are used and the recommended protocols are followed.
References
[1] Olivetti G, Anversa P, Rigamonti W, et al. (1977) J Cell Biol. 75(2 Pt 1):573-85.
[2] Couchman JR, Beavan LA and McCarthy KJ. (1994) Kidney Int. 45:328-35.
[3] Riser BL, Cortes P, Heilig C, et al. (1996) Am J Pathol. 148:1915-23.
[4] Gruden G, Thomas S, Burt D, et al. (1999) J Am Soc Nephrol 10:730-37.
[5] Wanner C, Greiber S, Kramer-Guth A, et al. (1997) Kidney Int Suppl 63:S102-6.
[6] Gomez-Guerrero C, Suzuki Y and Egido J. (2002) Kidney Int 62:715-7.
[7] Schl