Meningeal cells surrounding the brain participate actively in the normal development of the central nervous system. They play important roles in stabilizing the extracellular matrix of the pial surface, organizing the radial glial scaffold, laminating the cerebellar cortex. Selective pharmacological destruction of the meningeal cells during a critical ontogenetic period leads to specific malformation of both the cerbella cortex and dentate gyrus [1]. Grafts of meningeal cells, which are derived from meninges overlying the cerebral cortex in adult rat spinal cord lesion, promote axonal regrowth [2]. Additionally, in vitro studies showed that meningeal cells chemotactically orient the migration of immature neurons but not glial cells [3].
Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. No. SC2301) for the culturing of RMF in vitro.
Product Use
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice
Warranty
Cells are only warranted if ScienCell media and reagents are used and the recommended protocols are followed.
References
[1] Hartmann, D., Sievers, J. Pehlemann, F. W. and Berry, M. (1992) Destruction of meningeal cells over the medial cerebral hemisphere of newborn hamster prevents the formation of the infrapyramidal blade of the dentate gyrus. J. Comparative Neurol. 320:33-61.
[2] Franzen, R., Martin, D., Daloze, A., Moonen, G. and Schoenen, J. (1999) Grafts of meningeal fibroblasts in adult rat spinal cord lesion promote axonal regrowth. Neuroreport 10:1551-1556.
[3] Hartmann, D., Schulze, M. and Sievers, J. (1998) Meningeal cells stimulate and direct the migration of cerebellar external granule cells in vitro. J. Neurocytol. 27:395-409.