The keratocytes, or corneal fibroblasts, are highly specialized cells that are sandwiched between orthogonally arranged layers of collagen lamellae in the corneal stroma. They play a key role in maintaining the structure and transparency of the cornea, as they are the source of stromal collagen and proglycans. They also play important roles in corneal wound healing and tissue repair, and are known to undergo phenotypic transformations in wounds due to the influence of growth factors and cytokines [1]. Under normal conditions, the keratocytes in the adult cornea are relatively quiescent cells. In the event of corneal injury or trauma, however, the keratocytes differentiate into active, synthesizing cells and rapidly replace damaged stromal matrix. Cultured human keratocytes express functional IL-4Rs [2] and IL-17R [3] on the cell surface, suggesting that these cells may contribute to the role of IL-4 and IL-17 as mediators of allergic reactions in the cornea. Changes in gene expression were observed in keratocytes after interleukin-1 treatment, which provides important insight into gene expression and suggests novel therapeutic targets for the control of corneal inflammation.
Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. No. SC2301) for culturing RK in vitro.
Product Use
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice
Warranty
Cells are only warranted if ScienCell media and reagents are used and the recommended protocols are followed.
References
[1] Fini ME. (1999)