RabRMC from ScienCell Research Laboratories are isolated from human renal tissue. RabRMC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. RabRMC are characterized by immunofluorescence with antibodies specific to fibronectin and smooth muscle actin. RabRMC are negative for mycoplasma, bacteria, yeast and fungi. RabRMC are guaranteed to further expand for 10 population doublings under the conditions specified by ScienCell Research Laboratories.
Recommended Medium: It is recommended to use Mesangial Cell Medium (MsCM, Cat. no. SC4201) for the culturing of RabRMC in vitro. Product Use RabRMC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping: Dry ice.
Product sheet
References: [1] Olivetti G, Anversa P, Rigamonti W, Vitali-Mazza L, Loud AV. (1977) "Morphometry of the renal corpuscle during normal postnatal growth and compensatory hypertrophy. A light microscope study." J Cell Biol. 75: 573-85.
[2] Gruden G, Thomas S, Burt D, Zhou W, Chusney G, Gnudi L, Viberti G. (1999) “Interaction of angiotensin II and mechanical stretch on vascular endothelial growth factor production by human mesangial cells.” J Am Soc Nephrol. 10: 730-7.
[3] Gómez-Guerrero C, Suzuki Y, Egido J. (2002) “The identification of IgA receptors in human mesangial cells: in the search for "Eldorado".” Kidney Int. 62: 715-7.
[4] Abboud HE. (2012) “Mesangial cell biology.” Exp Cell Res. 318: 979-85.