Rabbit Iris Pigment Epithelial Cells (RabIPEpiC) are isolated from Dutch rabbit iris. RabIPEpiC are cryopreserved at passage one and delivered frozen. RabIPEpiC are characterized by immunofluorescence with antibodies specific to cytokeratin-18. RabIPEpiC are negative for mycoplasma, bacteria, yeast, and fungi. RabIPEpiC are guaranteed to further culture under the conditions provided; however, RabIPEpiC are not recommended for subculturing due to limited expansion capacity.
Recommended Medium: It is recommended to use Epithelial Cell Medium (EpiCM, Cat. no. SC4101) for culturing RabIPEpiC in vitro.
Product Use: RabIPEpiC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen, and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping: Dry ice.
References: [1] Schraermeyer U, Enzmann V, Kohen L, Addicks K, Wiedemann P, Heimann K. (1997) “Porcine iris pigment epithelial cells can take up retinal outer segments.” Exp Eye Res. 65: 277-87.
[2] Semkova I, Kreppel F, Welsandt G, Luther T, Kozlowski J, Janicki H, Kochanek S, Schraermeyer U. (2004) “Autologous transplantation of genetically modified iris pigment epithelial cells: a promising concept for the treatment of age-related macular degeneration and other disorders of the eye.” PNAS. 99: 13090-5.