The salivary gland produces enzyme-containing saliva to lubricate the oral cavity and assist with digestion. It consists of three pairs of major salivary glands and many minor ones in the oral cavity [1]. Histology studies show that the salivary gland is a compound tubuloalveolar gland, surrounded by connective tissue to further divide the gland into lobes and lobules. The secretory end pieces consist of a spherical mass of cells called acinus. Mouse salivary gland fibroblasts (MSGF) are embryonic mesoderm derived mesenchymal cells and they are responsible for synthesis of extracellular matrix such as type I and/or type III collagen [2]. MSGF are very versatile and provide a useful tool for studying collagenous fibroma and oral neoplasms [3].
Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. No. SC2301) for culturing MLF in vitro.
Product Use
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice
Warranty
Cells are only warranted if ScienCell media and reagents are used and the recommended protocols are followed.
References
[1] Bradley A (1987) ” Production and analysis of chimaeras”. In Robertson EJ, Teratocarcinoman and Embryonic Stem Cells: a practical Approach (pp 113-51). Oxford: IRL Press. [2] Nagy A, Gertsenstein M, Vintersten K, Behringer R (2006) ” preparing mouse embryo Fibroblasts”. Cold Spring harbor Protocols. Pdb.prot 4398.